Tissue Culture


 
 
Concept Explanation
 

Tissue Culture

Tissue culture involves maintaining of tissues or organs (in case of plant tissue culture isolated cells or small pieces of plant tissues are used) under sterile or  in vitro conditions in nutrient cultures to grow into new disease-free plants. This technique is also known as in vitro micropropagation because it takes place in a laboratory and the plants are transferred from the labs to the soil.

Plant tissue culture is widely used for the rapid multiplication of plant species with superior qualities. This method involves following steps:

  • In this method, small pieces are cut from the plant like flower buds, stem tissue, growing tips, leaves, etc., (known as ex-plants).
  • These a then transferred to an artificial medium where they divide rapidly to form a callus (undifferentiated mass of cells).
  • This callus is then transferred to fresh medium containing hormones for growth and differentiation, where it subsequently develops into platelets.After this, the developed platelets are placed in the soil to grow into a mature plant.
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    Sample Questions
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    Question : 1

    Cloning is the production of an exact copy of a cell, any other living part or a complete organism. A sheep named Dolly was the first mammal to be cloned by lan Wilmut and his collegaues at the Roslin Institute in Deinburgh. Find out the incorrect statements about cloning of Dolly.

    • A. Dolly was born on 14^t^h February, 1998.
    • B. Dolly was given birth by a Finn Dorest sheep.
    • C. Dolly was found to be absolutely indentical to the scottish blackface ewe.
    • D. The nucleus of the mammary gland cell from the Finn Dorestt sheep was insrted into the egg of the scottish blackface ewe whose nucleus and been removed.
    Right Option : C
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    Question : 2

    By which of the following methods can a disase free plant be produced ?

    Right Option : D
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    Question : 3

    Stem cuttings are commonly used for the propagation of ___________________

    Right Option : B
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